In Search and Do Not Destroy, the alligator has its first appearance in this series guarding the key that Leshawna is looking for to unlock her treasure chest. She managed to fight off three of them and successfully retrieved her key.
In Total Drama, Drama, Drama, Drama Island, while Duncan and Courtney tried to reel the suitcase filled with one million dollars back to shore, an alligator appears and eat the case. Duncan dive into the lake and ended up fighting with the alligator for the case. In the end, Duncan came out victorious and manage to get the alligator to spit out the case.
In Walk Like An Egyptian - Part 2, the contestants have to paddle in boats across the Nile River which is infested with crocodiles, while singing Rowin' Time. On Team Victory's boat, Harold advises Ezekiel to fend off the crocodiles by striking them on the nose. Ezekiel attempts to do so using a stick (which the team were supposed to have to avoid losing). To Ezekiel's dismay, the stick ends up getting eaten by the crocodile, and Team Victory loses.
In Broadway, Baby!, a giant albino alligator attacks all of the teams in the New York sewers. This alligator is significantly larger and lighter than the alligators seen so far in Total Drama. It ate Team Amazon's boat, but they managed to escape. Also, Chris ordered Chef to ask the alligator's signature for it to appear on television. In the exclusive clip, this alligator turns out to be DJ's pet named "Vince" who he accidentally flushed down a toilet when it was a baby after giving it a bath in the sink. Later, DJ goes down into the New York sewers and is reunited with his pet.
In The Treasure Island of Dr. McLean, a mutant alligator is seen attacking the contestants when they are in the bayou. As a result from the radiation, it now possessed two tentacles, replacing its front feet and has grown several times larger than a normal alligator. Luckily for the Mutant Maggots, Dakota (who also had mutated into a giant) fought off the alligator and defeat it. Two smaller ones (presumably the children of the giant one) are then seen attacking Lightning.
In Moon Madness, an alligator appeared in front of the frightened Villainous Vultures. Due to the Blue Harvest Moon, it wags it's tail instead of attacking them. Heather befriends the alligator and vigorously hugs it. In No One Eggspects the Spanish Opposition, a mutant alligator can be seen at the Fun Zone, stomping in the background.
In Sundae Muddy Sundae, an alligator by the name of "Snappy" appeared during the second part of the challenge, where it guarded a bowl of cherries. It attempted to attack Gwen but was knocked down by Mike (as Mal). The alligator then tried to eat Zoey, but she threw mud in it's mouth before it can get the chance. The alligator then gets into a fight with a bear.
In This Is The Pits!, while Max and Scarlett are traversing through a cave full of hazardous stalactites, Chris sends a crocodile to make things more difficult. Scarlett wishing to dispose of Max, suggests that he use his mental powers to take control of the crocodile. Max foolishly listen to her advice and is attacked by it. Luckily, a stalactite falls on the crocodile, revealing that it is a robot. Using its components, Scarlett manages to construct a universal remote control to help them in escaping the caves.
In Sky Fall, several crocodile robots to attack the remaining contestants as they climb up Mt. Chrismore. According to Chris, these robot crocodiles are more vicious than the previous model. They are shown being capable of flying via inbuilt rockets, and are installed with MyTunes. At the top of the mountain, the crocodiles attempts to stop Shawn from retrieving the flag and one of them successfully swallow him. However, he manages to break out of the crocodile by destroying it from the inside, winning the challenge. Meanwhile, Sky manage to land on one of them after she was pushed off the cliff by Sugar and direct it to lift her to the finish line. At the end of the episode, Sugar is seen applying make up on a crocodile.
In Ca-Noodling, Josee and MacArthur trick a blind Devin into thinking a crocodile is a large catfish. He brings the crocodile into his team's boat where it promptly eats all the fish they had caught. However, Brody uses the Heimlich maneuver to make the crocodile regurgitate all the fish, so they still end up completing the challenge.
- In Chomp Sewey, the player plays as the albino alligator from Broadway, Baby!. The objective is to eat all of the contestants in the boat. The regular alligator appears in the beginning on a refrigerator.
- Total Drama Action is currently the only season in which neither the alligator nor the crocodile appear.