In Not Quite Famous, it is revealed that Harold brought a red ant farm to Camp Wawanakwa. After Heather reads Gwen's diary aloud at the talent show, Gwen borrows the red ant farm and dumps it on Heather.
In This Is The Pits!, Jasmine awakes Max and tells him he is covered in ants. Max discovers his whole bed is infested with them and attempts to exterminate the colony by burning them with a magnifying glass, but only ends up setting fire to his bed.
In Brazilian Pain Forest, bullet ants play a role in the "Botch or Watch" challenge, where one member of each team must reach into an oven mitt full of ants to retrieve a Travel Tip. Most contestants get one hand bitten by the ants and remain swollen for the rest of the episode. Laurie is unfortunate enough to get knocked face-first into the oven mitt, resulting in her face swelling.
In Ant We All Just Get Along, the kids discover an anthill on the playground which is home to some ants Beth befriends. Leshawna and Courtney however, did not like the ants, and attempted to get rid of them. At the end of the episode, however, the kids send the ants faraway to where the couple's picnic ends up getting ruined by them.
In A Ninjustice to Harold, a fire ant breathes fire on Owen, causing him to go bald.
An armadillo makes a brief appearance in Bjorken Telephone, when Jay says Mickey's knees look like armadillos whenever he gets eczema. The scene shifts over to an actual armadillo blowing a raspberry, offended by the comparison.
The baboon only appears in African Lying Safari. It finds Cody trapped on the branch of a tree and captures him. Later, it gives Cody a foot massage, which he seems to enjoy. Once Sierra arrives however, the baboon and its family become violent and begin to screech at her. Sierra is jealous of the baboons spending time with Cody and attacks them, eventually knocking three of them unconscious and forcing Cody's captor to flee.
The bat first appears in The Sucky Outdoors, frightening Katie and Sadie in a cave after they are separated from their team. Later, another flies onto Bridgette's face, causing her to accidentally set fire to her team's tent.
In If You Can't Take The Heat..., when Owen is getting the oranges for the challenge, he accidentally attracts bees in a nearby hive above him, which viciously attack him. In No Pain, No Game, Bridgette needs to wear a shirt composed of live bees as a part of the challenge.
In Search and Do Not Destroy, Izzy helps Lindsay with the challenge resulting in her being covered in bees. The bees then chase Lindsay who runs straight to the dock, jumping off, in order to avoid the mob of bees. In I Triple Dog Dare You!, Owen must wear a beard made out of bees as a part of Eva's dare.
In Evil Dread, Scott accidentally releases an angry swarm of bees from one of the traps. In Food Fright, when Sam eats the pancakes, he doesn't notice he ate a beehive which contains a swarm of bees. When the challenge ends, Alejandro is stung by a bee in the cheek.
The blowfish is used in the extreme cooking challenge in Who Can You Trust?, when Lindsay and Trent are pitted against Bridgette and Geoff. According to Chris, the fish contains enough poison to kill at least thirty people, and therefore it must be sliced very carefully to cut around the poisonous organs. Bridgette is able to successfully prepare the blowfish in the form of sushi, but Lindsay is unable to do the same and ends up poisoning Trent.
The blowfish is next seen in Crouching Courtney, Hidden Owen when Beth and Courtney have to prepare a Japanese dinner for Harold, the winner of the reward challenge. This includes a dish called Kung Fu Noodle Soup, which is made of vegetable broth, noodles, and the "seven deadliest species of fish known to man" (including the blowfish). Beth claims she is allergic to fish when they're still alive, so she can't help Courtney cook it. Courtney cooks the dish incorrectly and Owen is poisoned after tasting the soup.
Butterflies occasionally appears throughout the series. In Total Drama, Drama, Drama, Drama Island, one can be seen flying around Justin's head. In Bigger! Badder! Brutal-er!, a butterfly landed on Cameron's head while he is using the confessional. As part of his running gag involving his weaker body structure, the butterfly proves to be heavy for Cameron to stay still when it landed on his head, resulting in pain for him. This scene is reused from Cameron's audition tapes. Later in the episode, a butterfly gets eaten by a mutant squirrel.
In None Down, Eighteen to Go - Part 1, a butterfly flies onto Miles' finger and then away from there during the Vegans' introduction. It reappears in El Bunny Supremo, as it passes by the interview room when Devin talks about his blindness.
Several contestants in the series such as Heather, Topher, and Jasmine own cats as pets. In Masters of Disasters, the cat is one of the many objects thrown by Chef. A cat appeared in Dial M for Merger, as Chris's pet who attacks him and as Chef's sidekick, Dander Boy, in Super Hero-ld.
Total Drama: Pahkitew Island introduces a mechanical kitten (known as cat-bot by Sky) that is able to shoot laser beams from its mouth. It first appears in Scarlett Fever attacking Sky and Sugar but they manage to run away from it. It also appears in finale at the first challenge end of the first part of the challenge. Upon noticing it, Sky taunts the cat to fire at her, melting the snowball that she and Dave are trapped in, resulting in Dave losing his hair.
Catfish are seen in Vietnam, where they are used in the challenge during Ca-Noodling. Contestants must catch six catfish using a traditional technique called "noodling", where they stick their hands in the water as bait for the fish to bite onto. The super alliance between the Best Friends, Sisters, and Surfer Dudes manage to easily catch ten catfish, while the "Axis of Evil" super alliance struggle due to Josee and MacArthur's fighting hindering the team.
The chameleon is introduced in Top Dog's animal buddy challenge, when Duncan chooses it. He does this in memory of his beloved pet, Scruffy, although he fails to remember that Scruffy is an arachnid and the chameleon is a reptile. He tries to train it to turn itself into the color of his mohawk. However, it fails several times. This is partly due to the fact that Owen kept sabotaging the pair by spray painting Duncan's mohawk a different color, so that the chameleon would change to match the color but get scolded by Duncan. By the end of the first challenge, the chameleon finally changes into the right color. In the second challenge, the chameleon is supposed to be Duncan's guide out of the woods. Duncan claims that chameleons are stupid animals and mentions that he should have picked the bear so he could hitch a ride back to the film lot without having to walk. The chameleon then camouflages with the scenery and Duncan believes it is missing for the rest of the episode. It does not reappear until Owen points out that Duncan and his chameleon are bonding, and it then reappeared on Duncan's head. Having blended in with his mohawk, Duncan then realizes it was on his head the whole time.
In Phobia Factor, it is revealed that Tyler has an irrational fear of chickens. The reason he is afraid of them, however, is never given. During his challenge, he couldn't last more than three minutes in the chicken pen. He even panicked to the point that he went into the fetal position, rocking back and forth. Tyler was later voted off for this and was sent on the Boat of Losers with tears in his eyes. Mainly, it was increasingly chicken related puns from his ex-teammates, only to be stopped by Courtney, that made him upset. He screams in horror when he realizes that the Boat of Losers is filled with chicken cages. It was most likely courtesy of Chris and Chef. In I Triple Dog Dare You!, when Heather is forced to act like a chicken, she has another chicken beside her.
In So, Uh This Is My Team?, when Beardo pulls his cord for his parachute pack, a chicken comes out. A chicken known as "Clucky" appears in I Love You, I Love You Knots. She is stated to be from the CIA, and can tell if someone is lying or telling the truth. She develops a conflict with Rodney after the latter mocks her, which leads her to take revenge by electrocuting him, resulting in Waneyihtam Maskwak winning the challenge, and Clucky being taken away in a straitjacket. Later, it is implied that Chef cooks Clucky for dinner. Clucky's design is different from the regular chickens that appear prior to this, as she didn't have dotted eyes. Future appearances of chickens would use this new design along with the older ones, the latter design also includes alternate colors.
In El Bunny Supremo, the final seven teams are forced to take a bus filled with chickens to Mexico. Though she didn't like the bus condition, Kitty still takes a selfie with one of chickens. In Darjeel With It, several chickens flew out of a train car after the Sisters break into it.
A chipmunk was first seen in the Total Drama Island opening sequence where it was among the animals relaxing with DJ until a dead fish falls from the sky which causes the chipmunk and the other animals to attack him. In Phobia Factor, a chipmunk scurries up to Bridgette while she is trying to conquer her fear of staying alone in the woods for six hours. When Bridgette sees the chipmunk, it frightens her, causing her to kick it out of the forest in fear. A chipmunk is briefly seen when Izzy's bike goes through a bush and it suddenly appears, desperately grabbing onto Leshawna's weave in an attempt to not fall off in That's Off the Chain!.
In Wawanakwa Gone Wild!, the chipmunk is Owen's animal to capture. It scurries away from him and runs up a tree, so Owen follows it. Owen tries making friends by talking in third person about his skills as a naturalist with the chipmunk and saying he loves it. However, the chipmunk bites him on the nose and Owen falls off of the tree branch he is on because he is too heavy. The chipmunk lands safely with a parachute, which it pulls out of nowhere. Owen eventually leads the chipmunk into the cage and screams at Chris to close the door. Chris asks if he is sure about that because when he closes the door, Owen is attacked by all of the animals including the chipmunk, because he locked himself in. In Total Drama, Drama, Drama, Drama Island, two chipmunks briefly appear, one fainting into the other chipmunk's arms, while the other fawns over Justin as he walks past them.
In Rock n' Rule, after Lindsay comes out of the shower and starts singing, some chipmunks get startled by her singing and run away from her. In the Total Drama World Tour Drop of Shame game, the chipmunk is seen on a parachute taking pictures throughout the game. In Awwwwww, Drumheller, the chipmunk is one of the many animals briefly seen running out of the Total Drama Jumbo Jet, before Sierra accidentally causes it to explode when she brings out Cody's birthday cake from plane.
The condor is seen in Rapa Phooey!. The eggs of the mother condor was used as part of the challenge. One of the eggs hatched and Sierra named the chick Cody Jr. After collecting the eggs, the contestants are tasked in placing the eggs back to the mother condor's nest while singing Condor. Eventually, Heather wins the challenge as the mother condor was being distracted by Cody Jr. The father condor also made a brief appearance chasing the contestant in the cave leading to the nest.
The dogs debut in I See London... as the guard dogs that protect the final clue in order to find Jack the Ripper. Gwen and Courtney see the dogs first and they growl ferociously at both of them. However, Gwen wins them over when she pretends to want to play ball with them by using a jewel encrusted egg, allowing the girls to receive their last clue, which was in the egg. Not long after, Owen and Noah run into the dogs, which then attack Owen. Owen doesn't seem to mind, as he does not show any signs of pain when they bite him all over his entire body, even joking that they looked like a fur coat. When Owen and Noah run into Jack the Ripper, Owen attempts to make the dogs attack the Ripper, but they attack him instead. One of the dogs manages to steal a sausage from Owen's pocket, which he accidentally kicks out of its mouth and into the Ripper's hands, prompting them to attack him instead. This allows Team Chris Is Really Really Really Really Hot to capture the Ripper. The dogs appear once more in a never-before-seen clip from Aftermath Aftermayhem, in the "That's Gonna Leave A Mark" segment, where several of them leap onto Noah in the confessional after he holds up a sausage.
A female corgi with a bow, similar to the guard dog corgis, appears on a podium during Blainerific, when Blaineley says that she was the hostess of "The Puppy Bachelorette".
In Brunch of Disgustingness, after Chris mentions the current meal was bull testicles, footage of a bull holding its groin area in pain is shown.
In Evil Dread, crabs are part of the traps that Chris has set up for the challenge. During the challenge, Sam is attacked by them. Jo later pushes Heather into a pit of crabs. A crab snaps at Alejandro's feet, and is soon after hit by Alejandro's shovel. Towards the end of the challenge, Lightning uses a crab as a substitute for a clock in the sculpture of Big Ben, causing the sculpture to collapse.
A cricket first appears in Beach Blanket Bogus, during the first challenge. It gets iced and it's leg fall off. It is later seen in Celebrity Manhunt's Total Drama Action Reunion Special after the original twenty-two contestants, plus Sierra are kicked out of the award ceremony. It starts making noises while a tumbleweed passes through. It is then crushed by Duncan, who is then scolded by DJ for killing it.
A deer makes its first appearance in Not So Happy Campers - Part 1, being given a noogie by Duncan. This incident is seen again in a flashback by Lindsay in Haute Camp-ture. In Wawanakwa Gone Wild!, Izzy is assigned to catch a deer for the challenge. She shoots everything with a tranquilizer gun, even Heather after mistaking her for a deer.
In Rock n' Rule, after Lindsay comes out of the shower and starts singing, a startled deer is seen running away from her.
In Slap Slap Revolution, after Tyler unintentionally causes an avalanche, Owen is seen riding a deer down the mountain and holding onto its antlers. In Hawaiian Style, Harold chooses a deer to represent Cody, for reasons that go beyond his "large, innocent eyes and tiny tail." During the challenge when Harold is about to place the lei on the deer, he misses and drops the lei. The deer eats the lei, causing him to receive second place in the challenge.
In The Final Wreck-ening, two deer, along with the other animals, can be seen riding the Boat of Losers after the island sinks at the end of the episode.
The dingo appears exclusively in Picnic at Hanging Dork, where Owen accidentally picks it up during a challenge in which he was supposed to catch a sheep; the dingo attacks Owen for this. Later, Duncan throws the dingo at Cody as revenge for his negative actions, and it is last seen entangled in a rope with Cody.
In Hawaiian Style, a doberman is one of the animals the eliminated contestants can choose to represent the final three they support. In the doberman's case, it represents Heather. While an intern ties it to a wooden raft, it roars at him, causing him to jump to the sea in fear. The challenge was to put a lei around the doberman but Owen and Blaineley fails, after the latter was launch too far from it.
In Justin's audition tape, a dolphin washes up on shore, attempting to be closer to Justin. In If You Can't Take The Heat..., the driver of the truck which delivered food was a dolphin with a delivery hat and suit on. It smiled and made a high-pitched sound when it arrived. The last disgusting meal the contestants were forced to eat in Brunch of Disgustingness were dolphin wieners, but DJ and Bridgette refused to, causing a different tie-breaker between the two teams.
A donkey named Ace is seen in Chinese Fake-Out. He is provided by Chris for the first challenge of racing across the Great Wall of China. Cody ends up using him for the challenge. During the second challenge, Cody is heard saying that he thought the first meal was Ace, and that he wouldn't eat it, because he had developed a bond with him.
In Hawaiian Honeyruin, contestants travel to their next location by riding in carts pulled by donkeys.
In El Bunny Supremo, contestants must ride donkeys, referred to as burros, to the Chill Zone. MacArthur experiences difficulty with her burro and ends up carrying it to the Chill Zone.
A duck appeared in Wawanakwa Gone Wild! where Gwen was assigned to catch it as part of the day's challenge. The duck was surprisingly fast, taking Gwen by surprise. In frustration, Gwen vented, "Last time I checked, ducks waddled." It is the first animal to be caught and due to this, Gwen wins the episode's reward. At the end of the episode, it attacks Owen along with all the other animals that had been captured.
Ducks appeared again in Total Drama: Revenge of the island in Runaway Model. Along the way to Boney Island, Sam begins to hallucinate two 8-bit ducks with targets on them. The duck is one of the few non-mutated animals in Total Drama: Revenge of the Island.
A duck makes an appearance in Moon Madness, where it gets affected by the Blue-Harvest Moon. It is first shown attacking Zoey, quacking demonically. Later it finds Cameron's discarded hat and puts it on, where it looks through the eye-holes on the hat.
The eagle makes its first appearance in the Total Drama Island opening sequence. After Owen knocks out a fish with his noxious fart, an eagle comes by, catches it and flies off. Later on in the sequence, the eagle drops the dead fish onto DJ's lap. This startles DJ and frightens the animals surrounding him in turn, so they attack him.
In Haute Camp-ture, an eagle grabs bunny and drops it in the barbecue.
During the scene where the bus is barely halted from hitting the ground in Celebrity Manhunt's Total Drama Action Reunion Special, a gopher sticks its head out of the ground and is shocked, but then relieved when the bus has halted. Moments later, it quickly gets picked up by an eagle, which flies off with it.
In Ice Ice Baby, an eagle swoops down to pick up a mutated rat, but the rat's eyes glow green and the eagle is tossed around telekinetically by the mouse.
In One Flu Over the Cuckoos, electric eels are present in the tank where the parts for the cadaver are located. Beth is discovers this the hard way, when she accidentally grabs an electric eel by mistake and gets electrocuted. Heather, DJ and Harold suffer this too.
In Jamaica Me Sweat, contestants must dive into a lake filled with electric eels while searching for gold chains. Tyler, Courtney, and Gwen all get electrocuted by the eels, the latter of which having such severe injuries she needs to be taken to the infirmary.
In A Blast from the Past, electric eels are present in Lake Pahkitew. Scarlett uses a jousting stick to grab one and passes it to Max so that he can electrify his jousting stick, who proceeds to electrocute Ella.
Emus first appear in Picnic at Hanging Dork, where the contestants have to capture and ride an emu up the famous Hanging Rock of Australia. The emus are fond of pecking and/or attacking the contestants and Chris' interns. Because of his weight, Owen is required to ride two emus and even so, both birds still couldn't carry him. As a result, Owen is the last person to reach the destination and by then, both emus are visibly exhausted.
In Last Tango in Buenos Aires, emus are used for the second challenge. Each team must catch an emu using boleadoras and carry it to the Chill Zone. Despite its presence, emus are not native to Argentina.
In Moon Madness, there appears a young fallow-deer, when Courtney is about to cross the finish line. Due to the Blue Harvest Moon's effects, its' behavior changed completely and it was ferocious. When it was going to attack Courtney, Gwen appears and puts a stick into its mouth. When the stick breaks, it is seen on Courtney and Gwen, ready to attack them, but the Blue Harvest Moon ends and it turns tame again and runs into the woods.
In The Final Wreck-ening it is seen at the end, in the boat of the animals that escape from the sinking island.
In Scarlett Fever it is one of the robotic animals that failed and were designated to protect the inside of the island, along with a bear, a deer and an alligator. It attacks Jasmine and Shawn, after being in contact with the lasers.
In Wawanakwa Gone Wild!, Leshawna is assigned the frog. Leshawna only takes a bucket from the boathouse, assuming she can capture the frog easily with it. However, it is not an easy task for Leshawna, as the frog irritates her by constantly luring her into unusually deep puddles instead of walking into the bucket. Leshawna begins to grow desperate in order to catch the frog, even trying to lure it towards her with cheese, which frogs don't eat. The frog still ignores Leshawna's pleas and forces her to come closer to her. Leshawna tries to ignore this trick, but eventually moves closer and ends up falling in the water again. Eventually, she captures the frog, (although it happens off-screen) and dumps it angrily into the cage. The frog later attacks Owen with all the other caged animals when he gets himself locked in the cage. The frog also makes an appearance in The Princess Pride, where it is kissed by Courtney, after she sings her song. The frog jumps out of her hands and lands on Justin's face, which makes him scream in terror at the thought of getting warts from it.
A frog appears in the Pahkitew Island opening sequence where it was sitting on a lily pad that had a camera underneath. A frog wearing sunglasses briefly appears sitting on Jen's head in French is an Eiffel Language.
The gopher is first seen in Celebrity Manhunt's Total Drama Action Reunion Special after the bus falls, in result of chasing the Total Drama Dirtbags' bus. It survived the bus crash thanks to Leshawna's bra, but it is then captured by an eagle.
Two normal gophers were seen in the Total Drama: Revenge of the Island opening sequence where they are amongst the many animals with Dawn, before they are scared off by a giant gopher bursting out of the ground.
A gopher is shown in a flashback in You Regatta Be Kidding Me, as Alejandro explains how he survived on Boney Island. He turns the bear against the gopher by complimenting the latter's eyes, causing the former to chase it.
They are also seen in This Is The Pits! as when Jasmine and Topher try to escape from underground, they find a tunnel that goes to the outside. The tunnel is made by gophers, and when they are about to attack them, Jasmine hits them with Topher's head, which allows them to reach the surface.
An iguana appears in I Love You, I Love You Knots, as part of a "scare" that Jasmine had to do; feed it with a scarab just using her mouth. The iguana wraps its tongue around her head, which causes her to run in fear. She gets a point for doing so.
The jaguar is seen only in Hawaiian Style. Courtney, without hesitation, chooses the jaguar as Alejandro's spirit animal saying that it perfectly suited his personality. Courtney later wins this challenge by putting a lei on the jaguar's neck.
The jellyfish made their first appearance during the second part of the three blind challenges in Who Can You Trust?. Four campers had to trapeze across a pond filled with jellyfish, in which two contestants, one on each team, were blindfolded. For the Killer Bass, Harold successfully caught a blindfolded Bridgette as she jumped to him. For the Screaming Gophers, Lindsay attempted to catch a blindfolded Heather as she jumped to her, but Lindsay mistimes the jump and Heather fell into the pond, resulting in Heather's trip to the infirmary. The second meal that the campers had to eat for the challenge in Brunch of Disgustingness was a pizza topped with jellyfish, grasshoppers, and live anchovies.
The jellyfish briefly appears for the first time in Total Drama Action in Crouching Courtney, Hidden Owen, when Courtney and Beth had to prepare a noodle soup consisting of the "seven deadliest fish known to man," of which the jellyfish was one. Courtney was seen fighting with a jellyfish, as she attempts to prepare the kung fu noodle soup.
In Picnic at Hanging Dork, after Cody hit Duncan while he was trying to get on his emu, Duncan charged at Cody in retaliation. His punch misses and he passes by, getting knocked flat by a kangaroo's kick. The kangaroo carries Duncan in its pouch up to Hanging Rock, and due to this Duncan is covered by slime when the kangaroo removes him. The kangaroo then proceeds to punch Duncan for mocking it. As the plane leaves Australia, the kangaroo is seen shaking its fist at the cast. In the exclusive clip for the same episode, it is shown kicking Gwen when she lands in the forest after her Drop of Shame. In Aftermath Aftermayhem, the kangaroo is seen once again, during Leshawna's challenge, where her goal was to defeat it in a boxing match in order to continue competing in the Second Chance Challenge. The kangaroo yawns repetitively in boredom, and knocks Leshawna out when Harold accidentally distracts her. Harold then proceeds to jump in the ring and avenge her, but the kangaroo knocks him out quickly and yawns once again in boredom.
The kangaroo reappears in Down and Outback as Chet and Lorenzo mistake it for an albino bunny, chasing it throughout the episode. By the time they fly towards the Chill Zone, the kangaroo gets on the Stepbrothers' glider and smashes one of the wings, causing it to crash in the snowy mountains of New Zealand. After the crash, the kangaroo chases the Stepbrothers, until they get buried by an avalanche.
In Newf Kids on the Rock, a killer whale appears underneath DJ and picks him up. While on the whale, DJ accidentally pushes the cushion into its blowhole, causing it to struggle. The whale manages to fling him into the boat and also shoots the cushion out of its blowhole, which hits a passing seagull.
In Picnic at Hanging Dork, Chris sets up a tie-breaker challenge for Courtney and Gwen in which they have to feed eucalyptus leaves to two hungry baby koalas. The koalas both attacked their respective contestant, scratching at their faces, but eventually they eat the food and become complacent.
Komodo dragons first appear in Got Venom, where contestants must collect their venom as part of the challenge. Several Komodo dragons chase the Police Cadets to the top of a tree, stranding them for a long duration. Owen wears a molted skin of a Komodo dragon to try and get closer, however one Komodo dragon becomes attracted to him, which Owen ends up naming Chewie.
The lion's first appearance is in African Lying Safari. During the challenge, when Heather was looking for Ezekiel, a lion appeared from behind the bushes, which caused Heather to run away terrified. While Chris was cutting for a commercial break, the lion scared him with his roar. The lion is also mentioned in Wake Up by Alejandro. In an exclusive clip for the episode, the lion is seen trapped in quicksand with Duncan right after he took his second Drop of Shame. They appear in Three Zones and A Baby, in the first zone to cross.
In Dude Buggies, a lion is used for the Magic challenge where one member of a team is trapped inside a cage with it while another must activate a trap door to make the lion "disappear." Because of their respective partners's unable to figure out the mechanism, Ryan and Jacques keep getting mauled by the lion. At one point, Loki jumps into the lion's mouth and Ennui had to pull his pet out of it, winning the challenge for his team. Stephanie on the other hand forces the lion into the trap door after having a scuffle with it. After an error made by Devin, Carrie falls into the trap door and had to defend herself from a pack of lions.
In Not So Happy Campers - Part 1, a loon is seen in the confessional stall putting on lipstick. It then notices that it was being filmed, and has a startled expression on its face until the confessional turns to static. In That's Off the Chain! it laughs at Heather after Lindsay calls Heather's shoes tacky.
Narwhals are present in the Arctic Circle, where they are featured as part of the challenge in Lord of the Ring Toss. Contestants must find rings and throw them onto a narwhal's horn to proceed. A narwhal later help the Cadets to reach the next challenge site by pushing the ice floe they are standing on.
Narwhals briefly appear at the beginning of Got Venom, where they are angered at being used in the challenge from the previous episode, and start puncturing the plane's tires, forcing the contestants to all board onto the one functional plane.
A much larger red octopus appears in the game Total Drama Freezer Burn, in which it will attack Alejandro on contact.
In One Flu Over the Cuckoos, a robot owl breifly appears in the beginning, hooing before malfunctiong and losing it's head.
In Saving Private Leechball, an owl is seen outside the Spa Hotel, hooing before the butler shushes it, making it hoo softer.
In Brazilian Pain Forest, an owl is stated by Don as one of the things in Brazil that keeps him awake at night, after which it screeches at the camera.
A pig appears briefly in I Triple Dog Dare You!, before Heather gets shot into feces.
In I Love You, Grease Pig!, grease pigs are used as batons the contestants need to pass for each obstacle course. Waneyihtam Maskwak's pig has a habit of farting and at one point, farts directly at Sky's face. It later was enchanted by Ella's song and begins dancing with her. Pimâpotew Kinosewak's pig on the other hand temporarily becomes evil after Scarlett places the helmet Max invented on it. After several other complications, Pimâpotew Kinosewak manages to reach the finish line first with their pig and wins the challenge.
In Cluckwork Orange, Beth was seen riding on a pig.
Piranhas first appear in No Pain, No Game, where Leshawna and a bear must stay on a log without falling into the piranha infested waters. Leshawna manages to knock the bear in, where its fur gets shaved off by the piranhas.
That's Off the Chain!, where there is a piranha pool that serves as an obstacle in the bike riding challenge.
In Backstabbers Ahoy!, Chef Hatchet throws Dakota into the lake, where she gets attacked by mutant piranhas.
Rats appear frequently during Total Drama Action and Total Drama World Tour. One is seen crawling around the space set in 2008: A Space Owen; another is seen chewing on a CD that Chris had sent Josh and Blaineley at the Celebrity Manhunt studio in Celebrity Manhunt's Total Drama Action Reunion Special.
Several rats live on the Total Drama Jumbo Jet; a few are seen parachuting out of the plane in Anything Yukon Do, I Can Do Better; in The Am-AH-Zon Race one ends up falling into Noah's mouth while he sleeps, but is immediately spat out. Another rat on the plane has its cheese stolen from it by Ezekiel in Niagara Brawls.
In the beginning of Bigger! Badder! Brutal-er! another mutant rat appears under one of the bed of one of the Cabins. It is shown to have a massive brain due to the radioactive waste. It is seen again at the beginning of Ice Ice Baby where it walks and stops at one point as it sees a eagle who is about to catch him until he uses his mind to pause it and slam it from side to side. It then goes in the main lodge through a hole.
It's then shown next to Cameron with the latter very excited to see it. He called it a "cute Apodemus sylvaticus". He was about to touch it until the rat flies in the air using his mind. All of the contestants then run out of the main lodge to avoid getting hit by a refrigerator with presumably the rat's mind. It is also possible that his mind also controlled Mike for a moment as he was shown not running with the others as well as getting thrown out and hitting the ground on his face.
In Food Fright, two rats are used as booby traps in the pancake eating challenge. They are seen attacking Alejandro. In Zeek And Ye Shall Find, Ezekiel uses two rats to chew on the rope Chris was hanging on. When Chef arrives to save Chris, he shoves the rats away.
In French is an Eiffel Language, a rat is seen running into a sewer whom the Surfer Dudes follow. Later, it scares the Fashion Bloggers with a large shadow.
In Soother or Later, Izzy pulls two rats out of a dumpster.
In My Way or Zimbabwe, as a part of the challenge, contestants must take a selfie with the rhino. Some contestants experience difficulty and get chased by the rhino, while others get their photo with little difficulty.
These small, carnivorous beetles are seen in Walk Like An Egyptian - Part 1 and Walk Like An Egyptian - Part 2 in Egypt. They emerge from a chamber in the ancient pyramid traversed by the contestants after DJ accidentally destroys a mummified dog he finds, indicating that they are a part of the Curse of the Mummified Dog.
The beetles attack and devour an intern and then prepare to attack the contestants, who then sing Lovin' Time in an attempt to soothe them into mating with each other rather than attacking them. The song works on them, but when Ezekiel ruins the song's rhythm, the scarabs become enraged and crawl towards the contests, who narrowly escape by fleeing. And they were seen again in the exclusive clip after Duncan took the Drop of Shame and his parachute gets stuck in a tree and scarabs are down there.
The scorpion makes an appearance in Walk Like An Egyptian - Part 1, stinging Tyler, when he is attempting to climb up the pyramid.
Sheep appear in Picnic at Hanging Dork during the second challenge, where the contestants must bungee jump off a cliff and grab one, shear it, and see if it has their team emblem on it. Only six of them have emblems, three for each team.
They also appear in Brazilian Pain Forest, where they were on the second plane with the second set of teams.
The snapping turtle is a reptilian animal that has a shell, into which it uses for defense. It also has a very powerful jaw. It is one of the many animals that appear throughout the Total Drama series.
In No Pain, No Game, Duncan's challenge from spinning the Wheel of Misfortune was to avoid the barrage of the nastiest snapping turtles in all of Camp Wawanakwa, while Chef Hatchet launches them at him with a hockey stick. Duncan manages to survive the required ten seconds, but not before being completely covered by turtles that were biting his entire body.
In Broadway, Baby!, during the Big Apple challenge, DJ volunteers to get the apple for his team, but is attacked by the turtles. When he finally manages to get away from them, there is still one is hanging on to his shirt. DJ pries it off, nicknaming it "Mr. Nibbles", and throws it back into the water, only for it to be eaten by a larger snapping turtle, which continues the running gag of DJ's Curse of the Mummified Dog.
In Aftermath: Revenge of the Telethon, during the "DJ's World of Animals" segment, meant to raise money for Total Drama, the clip of DJ making the snapping turtle get eaten by a larger snapping turtle is shown again. The turtle is also shown in a cage with the rest of the animals that DJ had hurt. When Blaineley releases all the animals in an attempt to get more money for the telethon, the turtle attacks Bridgette.
In The Bold and the Booty-ful, a single snapping turtle appears as a booby trap for Mal and it bites him on the hand. However, Mal growls at the snapping turtle and it ran off whimpering.
Stymphalian Canadian geese
These geese live in nests on the cliffs of Boney Island, where they attacked the Screaming Gophers. They eat bread, like any other goose, as seen in Up the Creek, when Cody throws bread at them and they begin to eat it and stop chasing them. They may be omnivores, as they eat the bread, but also attack the campers.
During the campers' time on the deserted island in Camp Castaways, Duncan finds a particularly large egg. Gwen finds out about this egg, and as they were both hungry they begin to fight over it. A goose then flies over them and cries out, implying that it is a goose egg. It then attacks Duncan to get its egg back and carries him off the ground. Gwen attempts to capture the goose by throwing a lasso around its leg, but the goose is easily able to lift Gwen off the ground as well. It later releases them for unknown reasons.
They reappear in One Million Bucks, B.C. where one steals Heather's new wig, attacked Harold at the end of the episode for "stealing" it's egg, and Heather attacking the one that stole her wig. These geese however, may have been animatronic.
In I Love You, I Love You Knots, Chef brings Sugar a tarantula on a platter for her dare. She picks it up and eats it before Chris has a chance to tell her she only has to pet it. Despite the unexpected turn, he decides to let it count as a point for the Waneyihtam Maskwak.
A toucan appears in The Am-AH-Zon Race.
In Jamaica Me Sweat, during DJ's final run at the longboard challenge, he ended up hitting several animals including a walrus when Alejandro reminded him of his curse. Finding it ridiculous that a walrus somehow ended up on the track, DJ yells that "It doesn't even make any sense!".
A wolf can be heard occasionally heard howling in the background at night during a few episodes of Total Drama Island. The silhouette of one can be seen usually before the Campfire Ceremony. In No Pain, No Game, the wolf is replaced with Chef Hatchet.
A wolverine is one of the animals MacArthur brought to the daycare in Cluckwork Orange. Cody pets the wolverine when MacArthur allows the children to play with the animals. From the wolverine, Cody's hand is contaminated with germs and he sucks his dirty thumb without washing it first, causing him to fall ill and later turned into a werewolf.
In Search and Do Not Destroy, while DJ was attempting to capture his key, the woodpecker is shown pecking at the branch, slowing DJ down.
A seemingly normal woodpecker was shown in Runaway Model trying to peck at Anne Maria's hair but failing to make any progress.